The waters acquity uplc iclass ensures fast analysis with high resolution power. Pdf curcuma aeruginosa is known as one of the components of herbal medicine. I am leaning towards water s xevo g2 xs qtof with uplc acquity hclass with pda or shimadzu qtof lcms9030. Adipose tissue was sampled using a prototype bipolar handheld sampling device connected directly to a xevo g2xs qtof system. In the ewre lab we have a watersmicromass xevo g2xs qtof, quadrupole timeofflight mass spectrometer. The xevo g2xs tof combines stepwave ion optics with the xs collision cell, providing a significant increase in sensitivity with no reduction in selectivity. Qtof 2 users guide micromass uk limited floats road. Wat introduces a new data acquisition mode, sonar, for its xevo g2xs quadrupole timeofflight qtof mass spectrometer.
View online or download waters xevo g2xs qtof overview and maintenance manual. Q tof 2 utilises a high performance, research grade quadrupole mass analyser, incorporating a prefilter assembly to protect the main analyser from contaminating. The quadrupole ion guide has a high mass cutoff of mz 16,000. The uplc analysis was performed on a waters acquity uplc system coupled to a waters xevo g2s qtof mass spectrometer waters co. Metabolomicsbased study reveals the effect of lead pb.
For positive mode, the cone gas flow was set at 20 lh. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc. Considerations specific to the xevo g2 qtof solventleakage hazard the source exhaust system is designed to be robust and leaktight. Q3, qtof or orbitrap lcms which will be better for. Here, we demonstrate apcs with a xevo g2 s q tof from watersmicromass using an ion source originally designed for atmospheric pressureelectron capture dissociation apecd experiments. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. What is the longest cable available to run between temperature controller 2 cn62 and the heater jacket on extraction vessel 2 for the biobotanical extraction system. The capillary voltage and cone voltage were set at 3.
Mass spectrometry was performed on a xevo g2s qtofms waters, operating in both negative and positive ion modes. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% lc eluate. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2 xs qtof provides maximum robustness with no compromise in performance, classleading realworld quantitative sensitivity, the highest quality, most. Use of liquid chromatography quadrupole timeofflight mass. This is designed for use with both gas and liquid sample inlet device, specifically a dedicated uplc and api gc. The xevo g2 xs qtof is for research use only and is not intended for use in diagnostic applications. Assessment of fruit juice authenticity using uplcqtof ms. We discuss advantagesdisadvantages of lcms for quantification and identification. The waters xevo g2 qtof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. Xevo g2 qtof laboratory equipment pdf manual download. Xevo g2 xs tof performance, robustness, and flexibility made accessible to everyone. Waters advances proteomics and lipidomics research with novel. Journal of pharmaceutical and biomedical analysis 142 2017 252261 253 to. Rapid evaporative ionisation mass spectrometry reims.
Comparison of different time of flightmass spectrometry modes for. Good laboratory practice, and consult your organization s safety representative for guidance. Advanced assessment of the physicochemical characteristics of. Uplcqtofmsbased phenolic profiling of melastomaceae. Quantof technology delivers superior uplccompatible mass resolution, matrixtolerant dynamic range, quantitative. Xevo g2 xs qtof extreme sensitivity, extraordinary selectivity, exceptional solutions. View and download waters xevo g2 qtof operator s, overview and maintenance manual online. During long analyses, critical hardware components stay clean for longer, so results are more reproducible and the laboratory stays productive. Watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comp rehensive qualitative and quantitative information.
A waters uplc h class coupled to a waters xevo g2xs qtof ms was used. Intended use of the xevo g2 xs qtof waters designed the orthogonal acceleration, timeofflight xevo g2 xs qtof for use as a research tool to deliver authenticated mass measurement. Restore the last known good settings from pm backup. Waters introduces xevo g2xs qtof and xevo tqs micro mass. These platforms were equipped with a twave collision cell, high energy collision dissociation hcd cell and iontrap collision. The xevo g2xs qtof combines sensitivity and selectivity with the mass accuracy, dynamic range and speed provided by quantof tm technology and the enhanced quantitative capability of tofmrm data acquisition. The glycan profiling analysis demonstrated a high degree of.
The xevo g2xs qtof combines stepwave ion optics, xs collision cell, and quantof. A novel methodology for in vivo endoscopic phenotyping of. Liquid chromatographymass spectrometry for the determination of. Waters advances proteomics and lipidomics research with. Milford, ma, usa, waters system in tandem with a quadruple time. The instrument is developed to give the most accurate and reproducible separation of complex sample matrices. Overview to scale the picture below is a simulation at scale of the waters xevo g2xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. Xevo g2xs qtof quadrupole timeofflight mass spectrometry. Tanshinones are active compounds that accumulate in the periderm, resulting in redcolored roots. Marys hospital london and the royal marsden hospital, uk. Xevo g2xs qtof extreme sensitivity, extraordinary selectivity, exceptional solutions. Xevo g2 qtof and xevo tqs tandem ms waters corporation were used. Metabolome and transcriptome analyses reveal quality change. Lc q tof ms analysis was performed on a waters xevo g2 s q tof mass spectrometer combined with acquity iclass liquid chromatography waters corp.
Anyone with real experience on either obitrap qe or xevo g2 s qtof, please, give me your suggestions, especially information for performance and stability, calibriation frequency, etc. In the ewre lab we have a watersmicromass xevo g2 xs qtof, quadrupole timeofflight mass spectrometer. The waters xevo g2 qtof is coupled to a waters acquity uplc i class, that offers the. Mass measurement the mass measurement accuracy of the instrume nt, in resolution mode, will be. The lc front end employed was either the acquity iclass or acquity mclass with the ionkey ms source.
View and download waters xevo g2 qtof operators, overview and maintenance manual online. Analyses were performed using waters xevo g2s quadrupole time of flight qtof, thermo q exactive qe and thermo ltqorbitrap mass spectrometers equipped with suitable uplc systems. May 2012 720004348en ao pdf ms sensitivity esi the peak at mz 503 from a solution of 500 pgl raffinose in 7030 acetonitrilewater no additives, will have an intensity of greater than 7,200 counts per second. The effect of cme on lipid and protein oxidation was investigated in. Ex vivo analysis was performed using a standard electrosurgery handpiece with aspiration of the electrosurgical aerosol to a xevo g2s iknife qtof mass spectrometer waters corporation.
Overview to scale the picture below is a simulation at scale of the waters xevo g2 xs qtof lcms mass spectrometer system right side connected with an acquity uhplc left side, installed on a ionbench ms mass spec bench or table. Antioxidant potential of chrysanthemum morifolium flower. The tof ms experiments were performed using a xevo g2s qtof ms system waters corp. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Comparison of different time of flightmass spectrometry. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2xs qtof provides maximum robustness with no compromise in performance, classleading realworld quantitative sensitivity, the highest quality, most. Qtof 2 utilises a high performance, research grade quadrupole mass analyser, incorporating a prefilter assembly to. Patients undergoing elective surgical resection for crc were recruited at st. For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples the xevo g2 xs tof provides maximum robustness with no compromise in performance, the highest quality, most comprehensive qualitative and quantitative information. Rapid evaporative ionisation mass spectrometry reims provides accurate direct from culture species identification within the. High resolution mass spectrometry quantitation explained.
Mass spectrometry systems, instruments, and technologies. Sep 14, 2011 watch this xevo g2 qtof animation to learn how you can obtain the highest quality, most comprehensive information that will enable you to make the right decisions quickly and confidently. The q tof 2 hybrid quadrupole time of flight mass spectrometer is available with electrospray ionisation esi and atmospheric pressure chemical ionisation apci. Uplcqtof ms conditio uplc water acquity hss c 18 150mm x 2 50c. However, lines with orange roots have been observed in cultivated fields. The xevo g2 xs qtof combines sensitivity and selectivity with the mass accuracy, dynamic range and speed provided by quantof tm technology and the enhanced quantitative capability of tof mrm data acquisition.
Jun 16, 2014 the waters xevo g2xs mass spectrometer, a new high performance benchtop quadrupole timeofflight qtof tm mass spectrometer, and the xevo tqs micro, a new and compact benchtop tandem. Here, we performed metabolome and transcriptome analyses to investigate the changes of orangerooted. The xevo g2 xs qtof combines stepwave ion optics, xs collision cell, and quantof technology for superior robustness, sensitivity, and selectivity along with dynamic range, mass accuracy, and speed of analysis, resulting in the highest quality, most comprehensive qualitative and quantitative information. Xevo g2s tof is designed for scientists who need to identify and quantify the broadest range of compounds in complex and challenging samples. Comparison of fragmentation experiments for the structural. The effect of cme on lipid and protein oxidation was investigated in goat patties during 9 days of refrigerated. Ionbench with the waters xevo g2xs qtof mass spectrometer.
Xevo g2xs tof performance, robustness, and flexibility made accessible to everyone. Metabolomicsbased study reveals the effect of lead pb in. Identification of methoxyisoflavone metabolites in human. I am leaning towards waters xevo g2xs qtof with uplc acquity hclass with pda or shimadzu qtof lcms9030. An optional high mass quadrupole ion guide is available with a high mass cutoff of mz 100,000.
The xevo g2 xs qtof delivers not only conventional ms and msms methods of data acquisition, but also mse for comprehensive accurate mass precursor and fragment ion information from a single analysis, fastdda for rapid, intelligent automated accurate mass msms, and tof mrm for enhanced quantitative capability. Chromatographic experiments were performed on a waters acquity uplc system connected to xevo g2 qtof ms equipped with an electrospray ionisation source waters corp. Considerations specific to the xevo g2 s qtof solventleakage hazard the source exhaust system is designed to be robust and leaktight. Please contact us for complete waters refurbishedused mass spectrometer system, warranty and service contract details.
The qtof 2 hybrid quadrupole time of flight mass spectrometer is available with electrospray ionisation esi and atmospheric pressure chemical ionisation apci. Anyone with real experience on either obitrap qe or xevo g2s qtof, please, give me your suggestions, especially information for performance and stability, calibriation frequency, etc. With regard to mass spectrometers, the igbb s proteomics staff utilizes a thermofisher ltq orbitrap velos, a waters nano esi q tof model xevo g2 s, and an applied biosystems now thermofisher maldi tof tof. Flavonoid and phenolic acid profile of chrysanthemum morifolium flower extract cme was analyzed by using ultra. For scientists who need to confidently identify and quantify unknown compounds in complex samples, the xevo g2 tof mass spectrometer with uplcmse and quantof technology offers exceptional levels of selectivity, inspectrum dynamic range, speed. Journal of pharmaceutical and biomedical analysis 142 2017 252261 contents. Lipidomics coupled with pathway analysis characterizes. Your quantitative and qualitative questions answered.
Data were processed using either masslynx or unifi. The ltq orbitrap velos and the nano esi q tof are fitted with upstream hplc sample purification systems. Which qtof is best abs triple tof5600 or waters g2s. May 10, 2019 mass spectrometry was performed on a xevo g2s qtofms waters, operating in both negative and positive ion modes.
The waters xevo g2 q tof is coupled to a waters acquity uplc iclass, that offers the most powerful solutions to complex separation challenges. Xevo g2 s qt of xevo tq s performance, robustness, and flexibility made accessible to everyone for the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples. Stepwave ion optics provides unsurpassed levels of durable sensitivity. The waters xevo qtof mass spectrometer offers high sensitivty, selectivity and dynamic. View online or download waters xevo g2 xs qtof overview and maintenance manual. Thermo persuades me to q exactive focus orbitrap lcms. Electrospray ionization esi source was used at positive ion mode.
Lipidomics coupled with pathway analysis characterizes serum. Connecting the epc in addition to a workstation pc or instrument server, the xevo g2 tof has an external epc with a realtime operating system that provides the realtime, dataprocessing required. Waters xevo g2xs qtof overview and maintenance manual pdf. Method of atmospheric pressure charge stripping for. Full scan and tof mrm mode of data acquisition were used. The end result is classleading quantitative performance in real everyday laboratory conditions, demonstrated by limits of detection and. Ms parameters were optimized via a direct infusion of methamphetamine and thccooh 500 ngml in the initial mobile phase separately at 10. Waters acquity hclass uplc with pda and column manager.
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